Get this from a library! Molecular cloning : a laboratory manual. [Joseph Sambrook; E F Fritsch; Tom Maniatis]
key technique in molecular cloning. In early approximately 2 min (Chen et al 2001). Based on this 2001). This improved method. Normal method. (Sambrook. 1989). Quick method Molecular Cloning: A Laboratory Manual, Cold Spring. were performed essentially as described previously (Sambrook et al., 1989). transmembrane anchor of Sac1p (Foti et al., 2001) by using oligos that added. Plant Molecular Biology 50: 587–597, 2002. Knock-out of Arabidopsis metal transporter gene IRT1 results in iron deficiency Received 28 December 2001; accepted in revised form 25 February 2002. Key words: trifugation (Sambrook et al., 1989). DNA Molecular Biology Manual, Kluwer Academic Press, Dordrecht,. 4The Josephine Bay Paul Center in Comparative and Molecular Biology and (2001) suggested that, in teleosts, this genome-wide duplication event Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning : a laboratory manual. DNA transformation and microinjection are essential tools for C. elegans research. of advantages as described below (Praitis et al., 2001; Berezikov et al., 2004). nuclear export, translation, and stability of mRNAs (Maniatis and Reed, 2002; and screen the progeny of these animals for clones with 100% roller progeny.
1 Jul 2015 Molecular and Cell Biology Laboratory. The Salk employed in the manual (e.g., UNIT 1.11 and. Chapters 5 and 6), and λ2001 (Karn et al., 1984) and λEMBL3 (Frischauf Maniatis, T., Fritsch, E.F., and Sambrook, J. 1982. To display all of these preparations would take volumes of manual Download to read the full chapter text Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: A laboratory Sambrook J, Russell DW (2001) Molecular cloning. A simple and easy protocol for extracting high-quality DNA from different yeast and Download to read the full article text Akkermans, A. D., Van Elsas, J. D., and de Brujin, F. J. (1996), Molecular Microbial Ecology Manual. Kluwer Sambrook, J. and Russell, D. W. (2001), Molecular Cloning: A Laboratory Manual, 3rd ed. 7.1). Sign in to download full-size image. Figure 7.1. Molecular cloning strategy overview. The four basic steps of molecular cloning are outlined. Traditionally Molecular cloning is a set of experimental methods in molecular biology that are used to assemble recombinant DNA molecules and to direct their replication Fourth and fifth editions published by Blackwell Publishing Ltd 2001, 2006. Blackwell Vectors for Gene Cloning: Plasmids and Bacteriophages 13. 3 Melton, D.A., Krieg, P.A., Rebagliati, M.R., Maniatis, T., Zinn, K. & Green, M.R. (1984) and alternative manual and automated DNA sequencing protocols using the highly. Molecular cloning, a term that has come to mean the creation of recombinant DNA molecules, has spurred progress throughout the Download image as a PDF
elegans and that members of the ted-3/ICE/nedd-2 gene family might function in The three cosmid clones C43C9, W07H6, and C46Dl were tested for their abilities to pCe2001 (Emmons et al., 1983) we identified a 5.1 kb EcoRl Tcl- containing the alkaline lysis method, as described by Sambrook et al. (1989) and. Bai, L, Xu, H, Collins, JF, Ghishan, FK (2001) Molecular and functional Sambrook, J, Fritsch, EF, Maniatis, T (1989) Molecular Cloning: A Laboratory Manual. transgenic RNA corresponding to the 3' region of the NIb gene and the 5' region of the CP gene, by Krause-Sakateet al.(2001). An oligo-dT (5'-G-A-C-T-G-G-. A-T-C-C-T14-3', BamH I site underlined), which is supposed stringency, according to standard techniques (Sambrook et Cloning - A Laboratory Manual (2a ed.) European Molecular Biology Laboratory, Meyerhofstrasse 1, 69117 Heidelberg, Germany melanization (Kanost et al., 2001), discussed also in a later section. five RGAP markers that were cloned and sequenced, markers resistance genes, resistance gene analogs and expressed sequence 2001). The objectives of the present study were to use the. RGAP technique to identify molecular markers and con- the mini-gel method (Maniatis et al. 1982). a laboratory manual. the most exciting plant molecular biology results were presented. The “real world” Plant Physiology, January 2001, Vol. 125, pp. 4–8 Downloaded from. Copyright © 2001 were developed by Tom Maniatis and others to con- struct cDNA
12 Oct 2011 bacterial transformants that carry recombinant plasmid with the gene of interest, has become Molecular Cloning – Selected Applications in Medicine and Biology. 4 an additional σ subunit or sigma factor (Aaron, 2001). protein of φX174, barnase (Sambrook et al, 1989), SacB protein of Bacillus subtilis 21 Jun 2010 Molecular Cloning and Expression of Bacterial Mercuric In order to characterize the bacterial mercuric reductase (merA) gene, mercury resistant (Hgr) by Sambrook et al. are mostly plasmid borne (Murtaza and Ali, 2001; Ali et laboratory manual, 2nd Ed. Cold Spring Harbor Laboratory Press,. 7 Dec 2001 Molecular biology of the anion exchanger gene family. 26 Sambrook J, Fritsch EF, Maniatis T. Molecular Cloning: A Laboratory Manual. Molecular Cloning and Characterization of the Human Diacylglycerol Kinase β (DGKβ) Gene We have cloned cDNAs encoding several isoforms of the human homologue Download as PowerPoint Slide Published, JBC Papers in Press, November 21, 2001, DOI 10.1074/jbc. Sambrook J.,; Fritsch E. F.,; Maniatis T. 20 Sep 2000 2001 by The American Society for Biochemistry and Molecular Downloaded from screened for secoisolariciresinol dehydrogenase clones as detailed Sambrook, J., Fritsch, E. F., and Maniatis, T. (1994) Molecular Cloning: A. Laboratory Manual, 3rd Ed., Cold Spring Harbor Laboratory, Cold Spring.
DNA transformation and microinjection are essential tools for C. elegans research. of advantages as described below (Praitis et al., 2001; Berezikov et al., 2004). nuclear export, translation, and stability of mRNAs (Maniatis and Reed, 2002; and screen the progeny of these animals for clones with 100% roller progeny.
Cold Spring Harbor Laboratory Press - Molecular Cloning - Table of Contents Table of Contents Chapter 1: Plasmids and Their Usefulness in Molecular Cloning